Proteinase K Powder 500mg | 507-PKP

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500 mg
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Proteinase K is an endoproteolytic enzyme that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a nonspecific serine protease. The smallest peptide to be hydrolysed is a tetrapeptide.

Applications: Proteinase K effectively digests native proteins. It can be used to inactivate DNases and RNases when isolating DNA and RNA from tissues or cells.

Usage Conditions:

• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5

• Under denaturing conditions—e.g., in the presence of SDS or urea

• In the presence of metal chelating agents—e.g., EDTA

• At high temperatures—optimum digestion temperature is 65°C

Unit Definition: One unit of the enzyme liberates folin-positive amino acids and peptides corresponding to 1μmol tyrosine in 1min at 37°C using denatured hemoglobin as substrate.

Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases

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3 Reviews

  • 5

    de confianza

    Posted by Aiden on 24th Jun 2022

    No hay duda de que este producto es increíble. Lo he probado y he visto grandes resultados con él.

  • 5


    Posted by Hunter on 24th Jun 2022

    Its employees are well mannered and the company's service is reliable

  • 5

    Fair reasonable prices

    Posted by Evan on 6th Jan 2022

    the price of their products is very fair and reasonable according to the quality they are maintaining in their products with no contamination

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