Proteinase K Powder 100mg | 505-PKP

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Proteinase K is an endoproteolytic enzyme that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a nonspecific serine protease. The smallest peptide to be hydrolysed is a tetrapeptide.

Applications: Proteinase K effectively digests native proteins. It can be used to inactivate DNases and RNases when isolating DNA and RNA from tissues or cells.

Usage Conditions:

• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5

• Under denaturing conditions—e.g., in the presence of SDS or urea

• In the presence of metal chelating agents—e.g., EDTA

• At high temperatures—optimum digestion temperature is 65°C

Unit Definition: One unit of the enzyme liberates folin-positive amino acids and peptides corresponding to 1μmol tyrosine in 1min at 37°C using denatured hemoglobin as substrate.

Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases

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Additional Information

100 mg
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3 Reviews

  • 5

    Posted by Ignacio on 24th Jun 2022

    He usado este producto y ha sido muy efectivo.

  • 5

    Posted by Jonathan on 24th Jun 2022

    Well-mannered employees with reliable service are the hallmarks of this company

  • 5

    Posted by Emmett on 6th Jan 2022

    their customer care and delivery of their products at a time with all the details on their site is outstanding

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