Proteinase K solution 25ml (20mg/ml) | 503-PK

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25 mL

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Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a serine protease. The smallest peptide to be hydrolysed is a tetrapeptide.

DNA Extraction lysis reagent for DNA tests & genotyping: The Proteinase K solution is useful when a small amount of biological samples are processed, and therefore when it is difficult to weigh Proteinase K powder. Add the enzyme solution to DirectPCR reagents at the final concentration of 0.5-1.0 mg/ml (25-50 μl Proteinase K solution per 1 ml DirectPCR reagent). Follow the instruction manuals for DirectPCR Reagent Protocol/Manual for each blood, blood stain, buccal swab, hair root DNA genotyping. Once diluted in DirectPCR reagents, Proteinase K is stable for ~24 hrs.

Other applications: Proteinase K digests native proteins very effectively. It can therefore be used to remove DNases and RNases when isolating DNA and RNA from tissues or cells.

Usage Conditions:

• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5

• Under denaturing conditions—e.g., in the presence of SDS or urea

• In the presence of metal chelating agents—e.g., EDTA

• At high temperatures—optimum digestion temperature is 65°C

Unit Definition: One unit of the enzyme liberates folin-positive amino acids and peptides corresponding to 1μmol tyrosine in 1min at 37°C using denatured hemoglobin as substrate.

Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases

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4 Reviews

  • 4
    good support

    Posted by Greyson on 22nd Jun 2022

    they provide an excellent support to their customers. Never delay or miss any complain and question.

  • 5
    imballaggio superbo

    Posted by Ginevra on 22nd Jun 2022

    Il modo in cui hanno confezionato i loro prodotti è eccezionale. Danno una copertura extra ai loro prodotti per proteggerli da danni fisici.

  • 5
    Envío rápido

    Posted by Isabella on 22nd Jun 2022

    Me gustaría agradecer a Genatur por su envío súper rápido y rápido. Debo decir que el personal de Genatur está bien capacitado y es responsable.

  • 5
    Quick shipping

    Posted by Declan on 6th Jan 2022

    Gentaur always fulfils its promise. They always shipped their products on time without any delay. Highly recommended!

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