Tribo™ Reverse Transcription Kit is optimized to synthesize first-strand cDNA for PCR or qPCR. The optimal first-strand cDNA synthesis temperature is 50°C. The reverse transcription reaction at higher temperatures improves cDNA yields for difficult RNA transcription. This pre-mixed 5X concentrated solution includes all necessary components for first-strand synthesis. With the mixture of 5X RT buffer, dNTPs, MgCl₂, DTT, primers (optimized mixture oligo (dT) and random primers), RNase inhibitors, and Reverse Transcriptase, the first-strand cDNA synthesis process is greatly simplified.

Applications:

This kit can be used for first-strand cDNA synthesis.

Key Features:

• Easy-to-use pre-mixed package (All in One)
• High fidelity, yield, and sensitivity
• Broader linear range
• Complete cDNA synthesis guaranteed
• Robust and high throughput
• Work with difficult sequences

Kit Contents:

5x RT reagent: 0.2mL
The kit contains RT buffer, dNTPs, DTT, RNase inhibitor, Primer mixer, and Reverse Transcriptase. The reagent is enough for 50 RT reactions in 20μL.

Storage Conditions:

The kit is shipped on ice. All components should be stored at -20°C in a non-frost-free freezer.
Shelf life is 12 months after receiving.