4-Methylumbelliferyl Phosphate (4-MUP) is a substrate for alkaline phosphatase that forms the soluble highly fluorescent reaction product methylumbelliferone. The 4-Methylumbelliferyl Phosphate (4-MUP) Liquid Substrate System combines 4-Methylumbelliferyl Phosphate (4-MUP) and buffer in a single solution, ready-to-use reagent for use in procedures detecting alkaline phosphatase enzyme activity in the culture media or ELISA. Fluorescence can be measured with excitation at 360nm and emission at 440nm. Also, the fluorescent end product may also be observed using a UV light source. This substrate is about 10 times more sensitive than the alkaline phosphatase substrate BCIP/NBT.

Applications:

• ALP substrate – for detection of alkaline phosphatase (alk-phos) activity on liquid system.
• Available for western blot.
• Custom packaging and bulk purchase information is available upon request.

Kit Contents :

Storage Conditions:

Supplied as a ready to use solution, 20mL. Store at 2-8℃. Protect from light for up to one year.