The 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) Substrate System is an ideal insoluble substrate for use with alkaline phosphatase. This system produces a blue-purple product. The intense color can be observed visually, is very stable, and will not fade upon exposure to light. The BCIP/NBT Substrate System offers a convenient, ready-to-use substrate solution for visualizing alkaline phosphatase activity in immunoblotting and immunohistology. Immunohistochemical staining with BCIP/NBT requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red.

Key Features:

• AP substrate: for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
• Chromogenic: no special equipment needed for visualization; produces purpleblack precipitate that is easy to photograph

Applications:

BCIP/NBT Solution is formulated to precipitate and localize the blue-purple reaction product onto membrane surfaces or tissue samples at sites where the alkaline phosphatase-substrate reaction occurs. BCIP-NBT Substrate produces a very large signal-to-noise ratio, although its high sensitivity requires careful washing and blocking to avoid background staining. When performing immunohistochemical techniques, organic mounting media must be used.

Storage Conditions:

Supplied as a ready to use solution, 2x 125mL (TBS5022-IHC & TBS5022-Blot).
Store at 2-8°C for up to one year.