CSFV Ab ELISA Kit

The BIONOTE CSFV Ab ELISA is a blocking Enzyme Linked Immunosorbent Assay for the qualitative detection of specific antibodies to Classical Swine Fever Virus(CSFV) which is the most common and prevalent virus, in Swine serum.
The BIONOTE CSFV Ab ELISA contains a microplate, which is pre-coated with recombinant E2(glycoprotein 55) on the well. For testing, ELISA plates are incubated with a serum and control(1:1 dilution with the sample diluents) for 60 minutes at 37℃. During first incubation, anti-CSFV antibodies present in sample bind onto the antigen coated on the well. After incubation and washing step, then mAb-HRP is dispensed into the wells and incubated for 30 minutes at 37℃. Following this incubation, all unbounded materials are removed by washing step. The enzyme linked to the complex is revealed by addition of a substrate. The enzyme activity will thus be in inverse proportion to the anti-CSFV antibodies in specimens and evidenced by incubating the solid-phase with a substrate solution for 15 minutes at room temperature(18~25℃). The reaction is stopped by adding stop solution, and colorimetric reading will be performed by using a spectrophotometer at 450nm and reference wavelength at 620nm.
The highly specific selected recombinant E2(gp55) antigens are used as capture material in test. These enable the BIONOTE CSFV Ab ELISA to identify to anti-CSFV antibodies in pig serum, with a high degree of accuracy.

CSFV Ab ELISA Kit Brochure

CSFV Ab ELISA Kit Insert