PRRS Ab ELISA Kit 4.0

Porcine Reproductive and Respiratory Syndrome (PRRS) is considered the most economically important viral disease of intensive swine farms in Europe and North America. The disease may also be referred to as Swine Infertility and Respiratory Syndrome (SIRS) by some veterinarians and swine industry professionals. The syndrome first began causing swine herd problems in the late 1980's and, prior to isolation of the causative agent, was often referred to as mystery swine disease.
The most commonly used serological tests for PRRS diagnosis are the indirect fluorescent antibody (IFA) test and enzyme linked immunosorbent assay (ELISA).
The BIONOTE PRRS Ab ELISA 4.0 contains a microplate, which is pre-coated with recombinant PRRSV antigen on the well. For testing, ELISA plates are incubated with samples and controls(1:39 dilution with the
sample diluents) for 30 minutes at room temperature(18~25℃). During
first incubation, anti-PRRSV antibodies present in sample bind to the antigen coated on the well. Following this incubation, all unbounded materials are removed by washing step. After that, rabbit anti-pig IgG-HRP is dispensed into the wells and incubated for 30 minutes at room temperature. The enzyme activity will be in proportion to the anti-PRRSV antibodies in sample and evidenced by incubating the solid-phase with a substrate solution. The reaction is stopped by adding a stopping solution, and colorimetric reading is performed using a spectrophotometer at 450nm and reference wavelength at 620nm.
The highly specific selected recombinant PRRSV antigens are used as capture material in this test. These enable the BIONOTE PRRS Ab ELISA 4.0 to identify to anti-PRRSV antibodies in sample, with a high degree of accuracy.

PRRS Ab ELISA 4.0 Brochure

PRRS Ab ELISA 4.0 Insert

PRRS Ab ELISA 4.0 Catalog