Mouse Arginase 1 (ARG1) ELISA Kit. Cat# abx051629. Supplier: abbexa.
Mouse Arginase 1 (ARG1) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Mouse Arginase 1 (ARG) concentrations in tissue homogenates, cell lysates and other biological fluids.
|Introduction||Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by ARG1 gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.|
|Target||Arginase 1 (ARG)|
|Recommended dilutions||Optimal dilutions/concentrations should be determined by the end user.|
|Storage||Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.|
|Validity||The validity for this kit is 6 months.|
|Stability||The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.|
|UniProt Primary AC||Q61176 (UniProt, ExPASy)|
|UniProt Secondary AC||Q3TB74, Q3UEL0, Q4FK78, Q80VI4|
|UniProt Entry Name||ARGI1_MOUSE|
|Test Range||15.6 pg/ml - 1000 pg/ml|
|Sample Type||Tissue homogenates, cell lysates and other biological fluids.|
|Kit Components||The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
|Material Required But Not Provided||
|Assay Procedure||This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
|Assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Interleukin 10 (IL10) were were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Interleukin 10 (IL10) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = (Standard Deviation / mean) × 100
|Availability||Shipped within 5-12 working days.|
|Note||This product is for research use only.
The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.
Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
|Plate coated with||Antibody|