Dylight 680, Rabbit Anti-Goat IgG | A23730

Product Overwiew:

Dylight 680 conjugated Rabbit Anti-Goat IgG(H+L) secondary antibody

Background:

Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples

The antibody reacts with whole molecule goat IgG. It also reacts with heavy chains of goat IgG, and light chains of all other goat immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.

Host:

Rabbit

Sequence:

N/A

Reactivity:

Goat

Application:

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1, 000 dilutions for most fluorescent applications, while 1:10, 000 dilutions for fluorescent assays with LI-COR® Odyssey equipment would be recommended.

Used for:

IF, ICC, FCM

Kit components:

N/A

Features & Benefits:

DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability. They are better than or comparable to the best fluorescent dyes from other companies. The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody. A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background. DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9.

Usage Notes:

N/A

Storage buffer:

Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative, 1% BSA as stablizer and 50% Glycerol.

Storage instructions:

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.