CheKine™ NAD/NADH Assay Kit | KTB1020

Product Overwiew:

Abbkine CheKine™ NAD/NADH Assay Kit is designed to detect the NAD and NADH, and their ratio in various tissues and cell.

Background:

Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP-ribosylation reactions and as a substrate for sirtuins.

Used for:

N/A

Kit components:

• Assay Buffer
• EtOH Solution
• MTT Solution
• PES Solution
• NAD Cycling Enzyme Mix
• NAD standard (10 mM)
• NAD Extraction Buffer
• NADH Extraction Buffer

Features & Benefits:

• Detect NAD+/NADH concentrations and ratios in cell or tissue extracts.
• Detection limit of 0.78 µM and linearity up to 50 µM NAD+/NADH in 96-well plate assay.

Usage Notes:

• Addition of Working Reagent should be quick and mixing should be brief but thorough.
• The following substances interfere and should be avoided in sample preparation. EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%).

Storage buffer:

N/A

Storage instructions:

Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.