CheKine™ NADP/NADPH Assay Kit | KTB1010

Product Overwiew:

Abbkine CheKine™ NADP/NADPH Assay Kit is designed to detect the NADP and NADPH, and their ratio in various tissues and cell.

Background:

Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms. NADP is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent. The oxidative branch of the pentose phosphate pathway (PPP) is the major source of NADPH produced in animal cells. There is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissues.

Used for:

N/A

Kit components:

• Assay Buffer
• Glucose (1 M)
• MTT Solution
• PES Solution
• NADP Cycling Enzyme Mix
• NADPH standard (10 mM)
• NADP Extraction Buffer
• NADPH Extraction Buffer

Features & Benefits:

• Detect NADP+/NADPH concentrations and ratios in cell or tissue extracts.
• Detection limit of 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.

Usage Notes:

• Addition of Working Reagent should be quick and mixing should be brief but thorough.
• The following substances interfere and should be avoided in sample preparation. EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%).

Storage buffer:

N/A

Storage instructions:

Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.