CheKine™ Peroxidase (POD) Assay Kit | KTB1060

Product Overwiew:

Abbkine CheKine™ Peroxidase (POD) Assay Kit is designed for the quantitative determination of POD activity.

Background:

Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ → ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues. Peroxidase (POD) conjugates are extensively used as secondary detection reagents in ELISAs, immunohistochemical techniques and Northern, Southern and Western blot analyses.

Used for:

N/A

Kit components:

• Assay Buffer
• Peroxidase Substrate
• Horseradish Peroxidase Standard (1 U/ml)

Features & Benefits:

• Determination of peroxidase activity in serum, plasma, tissue/cell lysates and other biological fluids.
• A broad range linearity: 15.6-1000 µU/mL.
• Quick POD assay in a one-step, homogeneous, no wash assay system.

Usage Notes:

• Bring all reagents to room temperature before using.

Storage buffer:

N/A

Storage instructions:

Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.