Human Anti-Granulocyte-Macrophage Colony Stimulating Factor antibody (GM-CSF-Ab) ELISA Kit | KTE62632

Product Overwiew:

This Human Anti-Granulocyte-Macrophage Colony Stimulating Factor antibody (GM-CSF-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate GM-CSF-Ab.

Background:

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Unlike granulocyte colony-stimulating factor, which specifically promotes neutrophil proliferation and maturation, GM-CSF affects more cell types, especially macrophages and eosinophils.

Host:

N/A

Sequence:

N/A

Reactivity:

Human

Application:

This Human Anti-Granulocyte-Macrophage Colony Stimulating Factor antibody (GM-CSF-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate GM-CSF-Ab in samples. An antibody specific for GM-CSF-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGM-CSF-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GM-CSF-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Used for:

ELISA

Kit components:

• Human Anti-Granulocyte-Macrophage Colony Stimulating Factor antibody microplate
• Human Anti-Granulocyte-Macrophage Colony Stimulating Factor antibody standard
• Human Anti-Granulocyte-Macrophage Colony Stimulating Factor antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits:

Human Anti-Granulocyte-Macrophage Colony Stimulating Factor antibody (GM-CSF-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human GM-CSF-Ab. No significant cross-reactivity or interference between Human GM-CSF-Ab and analogues was observed.

Usage Notes:

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage buffer:

N/A

Storage instructions:

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.