Human Anti-TNF-alpha autoantibody (TNFA-Ab) ELISA Kit | KTE62496

Product Overwiew:

This Human Anti-TNF-alpha autoantibody (TNFA-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFA-Ab.

Background:

TNFa is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-αis released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-αinclude B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.

Host:

N/A

Sequence:

N/A

Reactivity:

Human

Application:

This Human Anti-TNF-alpha autoantibody (TNFA-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFA-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated TNFA-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled TNFA-Ab and unlabeled TNFA-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TNFA-Ab in the sample. The color development is stopped and the intensity of the color is measured.

Used for:

ELISA

Kit components:

• Human Anti-TNF-alpha autoantibody microplate
• Human Anti-TNF-alpha autoantibody standard
• Human Anti-TNF-alpha autoantibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits:

Human Anti-TNF-alpha autoantibody (TNFA-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFA-Ab. No significant cross-reactivity or interference between Human TNFA-Ab and analogues was observed.

Usage Notes:

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage buffer:

N/A

Storage instructions:

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.