Human Interleukin 13 (IL-13) ELISA Kit | KTE62567

Product Overwiew:

This Human Interleukin 13 (IL-13) ELISA Kit employs a two-site sandwich ELISA to quantitate IL13.

Background:

Interleukin 13 (IL-13) is produced primarily by activated T lymphocytes. It shows approximately 30% amino acid (aa) sequence identity with IL-4 and shares many biological properties with IL-4. IL-13 has multiple effects on the differentiation and functions of monocytes/macrophages. It can suppress the cytotoxic functions of monocytes/macrophages and the production of proinflammatory cytokines by monocytes/macrophages. The biological effects of IL-13 are mediated by specific high-affinity cell surface receptor complexes. The functional IL-13 receptor complex has been shown to consist of the low-affinity IL-13 receptor α chain and the IL-4 receptor α subunit (IL-4 Rα). This IL-13 receptor complex may also serve as an alternate high-affinity IL-4 receptor complex in IL-4 responsive cells that lack the γ chain.

Host:

N/A

Sequence:

N/A

Reactivity:

Human

Application:

This Human Interleukin 13 (IL-13) ELISA Kit employs a two-site sandwich ELISA to quantitate IL13 in samples. An antibody specific for IL13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Used for:

ELISA

Kit components:

• Human Interleukin 13 microplate
• Human Interleukin 13 standard
• Human Interleukin 13 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits:

Human Interleukin 13 (IL-13) ELISA Kit has high sensitivity and excellent specificity for detection of Human IL13. No significant cross-reactivity or interference between Human IL13 and analogues was observed.

Usage Notes:

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage buffer:

N/A

Storage instructions:

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.