Human Nerve growth factor (NGF) ELISA Kit | KTE61336

Product Overwiew:

This Human Nerve growth factor (NGF) ELISA Kit employs a two-site sandwich ELISA to quantitate NGF.

Background:

NGF is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. Nerve growth factor has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in NGF have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.

Host:

N/A

Sequence:

N/A

Reactivity:

Human

Application:

This Human Nerve growth factor (NGF) ELISA Kit employs a two-site sandwich ELISA to quantitate NGF in samples. An antibody specific for NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Used for:

ELISA

Kit components:

• Human Nerve growth factor microplate
• Human Nerve growth factor standard
• Human Nerve growth factor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits:

Human Nerve growth factor (NGF) ELISA Kit has high sensitivity and excellent specificity for detection of Human NGF. No significant cross-reactivity or interference between Human NGF and analogues was observed.

Usage Notes:

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage buffer:

N/A

Storage instructions:

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.