Human Tissue inhibitors of metalloproteinase 1 (TIMP1) ELISA Kit | KTE60327

Product Overwiew:

This Human Tissue inhibitors of metalloproteinase 1 (TIMP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMP1.

Background:

TIMP metallopeptidase inhibitor 1, a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.

Host:

N/A

Sequence:

N/A

Reactivity:

Human

Application:

This Human Tissue inhibitors of metalloproteinase 1 (TIMP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMP1 in samples. An antibody specific for TIMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Used for:

ELISA

Kit components:

• Human Tissue inhibitors of metalloproteinase 1 microplate
• Human Tissue inhibitors of metalloproteinase 1 standard
• Human Tissue inhibitors of metalloproteinase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits:

Human Tissue inhibitors of metalloproteinase 1 (TIMP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TIMP1. No significant cross-reactivity or interference between Human TIMP1 and analogues was observed.

Usage Notes:

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage buffer:

N/A

Storage instructions:

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.