Mouse Interleukin 17 (IL-17) ELISA Kit | KTE71385

Product Overwiew:

This Mouse Interleukin 17 (IL-17) ELISA Kit employs a two-site sandwich ELISA to quantitate IL17A.

Background:

Interleukin 17 (IL-17; also known as IL-17A) is a 30 - 35 kDa variably glycosylated homodimeric protein that belongs to a unique family of cysteine-knot related proteins. Its sequence was originally isolated from an activated rodent hybridoma and termed CTLA-8 . It is synthesized as a 155 amino acid (aa) precursor that contains a 23 aa signal sequence and a 15 kDa, 132 aa mature segment. Although there are two intrachain disulfide bonds that create a ring reminiscent of those found in cysteine-knot proteins, the actual closed knot structure does not appear to form. IL-17 has one potential N-linked glycosylation site.IL-17, a T cell-derived proinf lammatory cytokine, is produced by TCRα/β+CD4-CD8- thymocytes, as well as by activated CD4+ and CD4+CD45RO+ memory T cells.

Host:

N/A

Sequence:

N/A

Reactivity:

Mouse

Application:

This Mouse Interleukin 17 (IL-17) ELISA Kit employs a two-site sandwich ELISA to quantitate IL17A in samples. An antibody specific for IL17A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL17A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL17A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL17A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Used for:

ELISA

Kit components:

• Mouse Interleukin 17 microplate
• Mouse Interleukin 17 standard
• Mouse Interleukin 17 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits:

Mouse Interleukin 17 (IL-17) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL17A. No significant cross-reactivity or interference between Mouse IL17A and analogues was observed.

Usage Notes:

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage buffer:

N/A

Storage instructions:

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.