Dihydroethidium (Hydroethidine) | BMD00001

Product Overwiew:

Dihydroethidium (Hydroethidine) is the reduction form of commonly used DNA dye-ethidium bromide.

Background:

The superoxide indicator dihydroethidium, also called hydroethidine, is the reduction form of commonly used DNA dye-ethidium bromide. It can be used to detect the inside living cells oxidation activity and detect phagocytes outbreak of "breathing". It exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red.

Used for:

N/A

Kit components:

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Features & Benefits:

• It exhibits blue-fluorescence (λEx/λEm:355/420nm) in the cytosol.
• It intercalates within the cell's DNA, staining its nucleus a bright fluorescent red (λEx/λEm: 518/605nm).
• In cells, hydroethidine is mainly oxidated by ROS, using flow cytometry instrument or fluorescence microscope can be directly observed, which is a kind of quick and easy classic method to detect ROS in tissue or living cells.

Usage Notes:

• Soluble in DMSO or DMF.
• The recommended work concentration is 1-10 μM when detect superoxide anion (O2-) in the cell.
• Prepare and use solutions on the same day.
• The stock solution should be used promptly. Any unused solution need to be aliquoted and frozen at < -20°C.
• Avoid repeated freeze-thaw cycles, and protect from light.

Storage buffer:

N/A

Storage instructions:

Store at -20°C. The product can be stored for up to 12 months. Protect from light.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.