Normal Human Serum | BMS0060
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High quality Normal human serum processed from non-immunized normal adult humans is used as a blocking agent or as a negative control for immunoassays.
Normal human serums, which are from non-immuned human hosts, are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide. Normal serum diluted to 5%-20% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific binding, or control for most immunoassay applications. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-p (1:10-20), IF/ICC (1:10-20) and ELISA (1:100).
IHC, ICC, IF, ELISA, BL
Features & Benefits:
10 mM sodium phosphate, 0.15 M sodium chloride with 0.05% sodium azide as preservative, pH 7.2
Store at 2-8°C or aliquot and store at or below -20°C for long-term storage. Avoid repeated freezing and thawing.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.