Description
Product Overwiew:
Abbkine Prestained Protein Marker (15-130kDa)
Background:
Protein marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents.
Used for:
N/A
Kit components:
N/A
Features & Benefits:
• Optimized for SDS-PAGE and Western blotting.
• Prepared in 1×SDS-PAGE loading buffer. Use directly without boiling, diluting or adding reducing agent.
• Suggested volume of the Marker onto the gel: Mini-gel, 2-5µL per well (0.75-1.0mm thick or 1.5mm thick). Large gel, 5-10µL per well (0.75-1.0mm thick or 1.5mm thick).
Usage Notes:
Thaw at room temperature. Mix gently and thoroughly to ensure that the solution is homogeneous.
Storage buffer:
Dye-stained proteins in 67mM Tris-H3PO4, pH7.5, 5mM EDTA, 2% (W/V) SDS, 33% (V/V) Glycerol, 0.02% (V/V) proclin300.
Storage instructions:
Stable for at least one year at -20°C or up to six months at 4°C from date of shipment. Aliquot to avoid repeated freezing and thawing.
Shipping:
Gel pack with blue ice.
Precaution:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.