ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit | KTP3001

ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit | KTP3001 from Abbkine.

Product Overwiew:

Abbkine ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue.

Background:

The preparation of an extract from nuclei is often the first step in studying nuclear proteins and their interactions. The resulting preparation can be used directly in the Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins.

Used for:

N/A

Kit components:

• Cytoplasmic Solution A (CES A)
• Cytoplasmic Solution B (CES B)
• Nuclear Extraction Solution (NES)
• DTT (500X)
• Protease Inhibitor (100X)

Features & Benefits:

• Versatile—suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations.
• Fast and convenient—non-denatured, active proteins are purified in less than two hours.
• Compatible—apply in various downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays.

Usage Notes:

Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.

Storage buffer:

N/A

Storage instructions:

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.