Human IL- 12 (IL-12A & IL-12B) protein, His Tag | PRP100705

Product Overwiew:

Recombinant Human IL- 12 (IL12A & IL12B Heterodimer) Protein (Interleukin-12 subunit alpha & Interleukin-12 subunit beta) expressed in Human Cells.

Background:

IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis.IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. There also seems to be a link between IL-2 and the signal transduction of IL-12 in NK cells. IL-2 stimulates the expression of two IL-12 receptors, IL-12R-β1 and IL-12R-β2, maintaining the expression of a critical protein involved in IL-12 signaling in NK cells. Enhanced functional response is demonstrated by IFN-γ production and killing of target cells.

The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method

Host:

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Sequence:

Amino acid sequence derived from p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met 1-Ser 219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; Amino acid sequence derived from p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

Reactivity:

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Application:

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Used for:

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Kit components:

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Features & Benefits:

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Usage Notes:

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL- 12 (IL12A & IL12B Heterodimer) protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage buffer:

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Storage instructions:

Lyophilized Human IL- 12 (IL12A & IL12B Heterodimer) protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Shipping:

The product is shipped at ambient temperature.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.