Human M-CSF protein | PRP100268

Product Overwiew:

Recombinant Human M-CSF Protein (Macrophage colony-stimulating factor 1) expressed in Human Cells.

Background:

Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

The Endotoxin level is less than 1.0 EU per μg protein as determined by the LAL method.

Host:

N/A

Sequence:

Amino acid sequence derived from human CSF1 (NP_757349.1) (Met1-Asn190) was expressed with an initial Met.

Reactivity:

N/A

Application:

N/A

Used for:

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Kit components:

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Features & Benefits:

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Usage Notes:

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human M-CSF protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage buffer:

N/A

Storage instructions:

Lyophilized Human M-CSF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Shipping:

The product is shipped at ambient temperature.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.