PurKine™ Protein L Packed Column 4FF | BMC20804

Product Overwiew:

PurKine™ Antibody Purification Protein L Packed Column 4FF

Background:

PurKine™ Protein L Purification system is effective for affinity purification of mammalian IgG that contain specific kappa light chains from serum, ascites fluid, cell culture supernantant and other antibody samples. Recombinant Protein L is chemically modified with a proprietary method to minimize nonspecific binding of proteins. Protein L is extremely useful for purification of VLk-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobilins, which are present in the media serum supplement. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Protein L Purification Resin is also available as prepacked spin column and kit formats.

Used for:

N/A

Kit components:

N/A

Features & Benefits:

• High capacity—More than 15mg human lgG per mL of resin.
• High performance—Recombinant Protein L is produced in E.coli.It binds Igs through interactions with the light kappa chains.Protein L binds a wider range of Ig classes than Protein A or G. Single chain variable fragments (ScFv) and Fab fragments also bind to Protein L.
• Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
• Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
• Robust—highly crosslinked beads tolerate linear flow rates up to 300cm/hour.

Usage Notes:

N/A

Storage buffer:

Liquid in PBS buffer containing 20% ethanol.

Storage instructions:

Stable for one year at 2-8°C from date of shipment. Do not freeze.

Shipping:

Blue ice

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.