abm | Fat-1 Stable HeLa Cell Line | T6260

abm | Fat-1 Stable HeLa Cell Line | T6260

Omega-3 polyunsaturated fatty acids (ω3-PUFAs) are known to inhibit carcinogenesis, tumour growth and metastasis, whereas omega-6 polyunsaturated fatty acids (ω6-PUFAs) stimulate their effects. The FAT1 gene of C. elegans encodes a ω3-desaturase that catalyses the conversion of ω6-PUFAs to ω3-PUFAs, increasing the amount of ω3-PUFAs. Fat-1 Stable HeLa Cell Line expresses the full-length FAT1 gene from C. elegans through stable transfection. These cells are applicable for studies investigating the effects of endogenous ω3-polyunsaturated fatty acids.

Biosafety:

II

Organism:

Human

Source Organ:

Cervix

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

20 - 30 hours

Seeding Density:

20,000 cells/cm²

Markers:

FAT1, G418 resistance (0.8 mg/mL)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) RT-PCR to detect FAT1 gene

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C