abm | HeLa Overexpressing Stable tCD-Luc2 Cell Line | T6023

abm | HeLa Overexpressing Stable tCD-Luc2 Cell Line | T6023

The HeLa Overexpressing Stable tCD-Luc2 Cell Line contains the fusion gene tCD2-luc2 from the luciferase 2 protein and the rat CD2 protein. The overexpression of tCD2-luc2 in the HeLa cells was done by stable transfection using lentiviral infection with the plasmid DNA containing the fusion gene. The growth of this cell line is identical to the wild type cells which indicates that the exogenous protein tCD2-luc2 has no toxic effect. The HeLa Overexpressing Stable tCD-Luc2 Cell Line shows a significant amount of luciferase activity which produces bioluminescent cells. This is useful as an experimental tool of major importance for cancer investigation, especially imaging of tumors in xenografted animals where stable GFP, labelling of tumor cells have to be avoided.

Biosafety:

II

Organism:

Human

Source Organ:

Cervix

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

13 - 28 hours

Seeding Density:

10,000 - 20,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

tCD2-luc2 gene introduced via lentiviral infection

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Flow cytometry on the basis of OX34 staining; 2) Western blot analysis of tCD2-luc2

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C