abm | Immortalized Chicken Skeletal Muscle Cells - SV40 | T9007

abm | Immortalized Chicken Skeletal Muscle Cells - SV40 | T9007

Immortalization Method:

SV40 antigen

BioSafety Level:

II

Organism:

(Gallus gallus) Chicken

Species:

Chicken

Source Organ:

Skeletal Muscle

Organ Type:

Skeletal Muscle

Growth Properties:

Adherent

Morphology:

Other

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 20% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C