abm | Immortalized Equine Kidney Cells (extEqFK) | T0084
Infected with non-replicating retrovirus containing a supernatant from the PA317 LXSN HPV16E6E7
5-month old Equine Fetus
40, 000 cells/cm10² to 50, 000 cells/cm10²
The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 5%, 1% L-glutamine , 600 µg/ml G418 (G271), and Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
The addition of G418 should be added only after cells initially recover from thawing.
1) Selected cells with presence of 600 µg/ml G418, 2) PCR analysis for HPV16 E6/E7 presence
liquid nitrogen or -180C