abm | Immortalized Fetal Bovine Lung Cells - SV40 | T0091

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SKU:
T0091
Availability:
5 to 7 Days Shipment
Size:
1x10⁶ cells / 1.0 ml
Quantity:
Inquire
€750.00
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Description

abm | Immortalized Fetal Bovine Lung Cells - SV40 | T0091

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Bovine

Species:

Bovine

Source Organ:

Lung

Organ Type:

Airway/Lung

Growth Properties:

Adherent

Morphology:

Other

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 20% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C

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