abm | Immortalized Sheep Kidney Proximal Tubule Cells - SV40T | T0902

abm | Immortalized Sheep Kidney Proximal Tubule Cells - SV40T | T0902

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen.

BioSafety Level:

II

Organism:

Sheep

Species:

Sheep

Source Organ:

Kidney

Organ Type:

Proximal Tubule

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV. to a final concentration of 10% 2mM L-Glutamine , 5µg/ml Insulin , 5µg/ml Transferrin , 1% Non-essential amino acids, 50nM Hydrocortinsone,and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

N/A

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C