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abm | Immortalized Preadipocyte Clonal Cell Line PGF2-8; Type 1 | T0803

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SKU:
T0803
Availability:
5 to 7 Days Shipment
AUD4,173.00

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Description

abm | Immortalized Preadipocyte Clonal Cell Line PGF2-8; Type 1 | T0803

Immortalization Method:

Conditionally immortalized. Cells were derived from the Immortomouse and grown at 33°C with 10 units/ml interferon-gamma.

BioSafety Level:

II

Organism:

Mouse (M. musculus)

Species:

Mouse

Source Organ:

Adipose

Organ Type:

Adipose

Growth Properties:

Adherent

Morphology:

Epithelial-like

Passage Number:

N/A

Population Doubling:

30 - 40 hours

Seeding Density:

10,000 - 20,000 cells/cm2

Markers:

Characterization markers (qPCR): Wt1, Lrn4, Upk3b.

Donor Age:

N/A

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, GlutaMAX (Gibco) to a final concentration of 2mM, recombinant mouse IFNG to a final concentration of 10 U/ml and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 33.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) qPCR; 2) Oil Red O staining

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C

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Additional Information

Size:
1x10⁶ cells / 1.0 ml
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1 Review

  • 4
    good support

    Posted by Zion on 22nd Sep 2022

    they provide excellent support to their customers. Never delay or miss any complaints and questions.

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