Description
abm | Immortalized Preadipocyte Clonal Cell Line PGF3-2; Type 2 | T0808
Immortalization Method: Conditionally immortalized. Cells were derived from the Immortomouse and grown at 33°C with 10 units/ml interferon-gamma. BioSafety Level: II Organism: Mouse (M. musculus) Species: Mouse Source Organ: Adipose Organ Type: Adipose Growth Properties: Adherent Morphology: Fibroblast-like Passage Number: N/A Population Doubling: N/A Seeding Density: N/A Markers: Characterization markers (qPCR): Tagln, Ctgf, Krt19 Donor Age: N/A Donor Gender: Male Donor Ethnicity: N/A Propagation: The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, GlutaMAX (Gibco) to a final concentration of 2mM, recombinant mouse IFNG to a final concentration of 10 U/ml and Penicillin/Streptomycin Solution to a final concentration of 1%. Quality Control: 1) qPCR; 2) Oil Red
O staining Shipping Condition: Dry Ice Storage Condition: liquid nitrogen or -180C
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 33.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.