abm | Immortalized Human Adipose-derived Stromal Cells - Bmi-1/hTERT | T0540

abm | Immortalized Human Adipose-derived Stromal Cells - Bmi-1/hTERT | T0540

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying mouse Bmi-1 (pLOX-CWBmi1) [Ref. seq.: NM_007552.4] and hTERT (pLOX-TERT-iresTK) [Ref.seq.: NM_198253.2] gene

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Adipose Tissue

Organ Type:

Adipose

Growth Properties:

Adherent

Morphology:

N/A

Passage Number:

N/A

Population Doubling:

90-100 Hours

Seeding Density:

6,000-8,000 cells/cm10²

Markers:

CD44, CD73, CD90 and CD105

Donor Age:

30

Donor Gender:

Female

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, 2mM L-glutamine and 50 ug/ml gentamicin . Optional for better growth: 1-5 ng/mL human recombinant bFGF .
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1. Real Time PCR, immunofluorescence, and telemorase activity was used to quantify Bmi-1 and/or hTERT gene expression in the immortalized cell line. 2. Culture tested negative for mycoplasma

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C