abm | Immortalized Human Cerebral Microvascular Endothelial Cells - Ras | T0262
- SKU:
- T0262
- Availability:
- 5 to 7 Days Shipment
- Size:
- 1x10⁶ cells / 1.0 ml
Description
abm | Immortalized Human Cerebral Microvascular Endothelial Cells - Ras | T0262
Immortalization Method:
Serial passaging and transduction with recombinant lentiviruses carrying Ras gene
BioSafety Level:
II
Organism:
Homo sapiens
Species:
Human
Source Organ:
Brain
Organ Type:
Brain
Growth Properties:
Adherent
Morphology:
Polygonal
Passage Number:
N/A
Population Doubling:
N/A
Seeding Density:
10,000 - 30,000 cells/cm10²
Markers:
N/A
Donor Age:
N/A
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow I medium available at abm. to a final concentration of 10%, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
Real Time PCR was used to quantify Ras gene expression in immortalized cell line.
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C