abm | Immortalized Human Corneal Epithelial Cells (HCE-S) | T0737

abm | Immortalized Human Corneal Epithelial Cells (HCE-S) | T0737

Immortalization Method:

Spontaneous

BioSafety Level:

II

Organism:

Human

Species:

Human

Source Organ:

Eye

Organ Type:

Eye

Growth Properties:

Adherent

Morphology:

Epithelial

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

Pancytokeratin, cytokeratin 3, ABCG2, α9 integrin, PAX 6, β1 integrin

Donor Age:

N/A

Donor Gender:

Female

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Scanning/transmission electron microscopy analysis; 2) Immunocytochemistry; 3) RT-PCR; 4) Western blot; 5) Scratch wound/proliferation/migration assay

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C