abm | Immortalized Human Coronary Artery Endothelial Cells | T0551

abm | Immortalized Human Coronary Artery Endothelial Cells | T0551

Immortalization Method:

Immortalized via infection with lentivirus expressing SV40 large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Heart

Organ Type:

Blood Vessel

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

5,000 - 10,000 cells per cm10²

Markers:

Dil-Ac-LDL uptake, vWF, Factor VIII-related antigen, CD31, puromycin resistance

Donor Age:

89

Donor Gender:

Female

Donor Ethnicity:

Caucasian

Propagation:

The base medium for this cell line is Prigrow I medium available at abm. to a final concentration of 20%, Penicillin/Streptomycin Solution to a final concentration of 1%, Recombinant Human EGF to a final concentration of 5 ng/mL, Recombinant Human FGF2 to a final concentration of 10 ng/mL, Recombinant Human VEGF (165aa) to a final concentration of 0.5 ng/mL, heparin to a final concentration of 90 μg/mL, and hydrocortisone to a final concentration of 0.2 μg/mL.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify the transgene expression.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C