abm | Immortalized Human Coronary Artery Smooth Muscle Cells | T0557

abm | Immortalized Human Coronary Artery Smooth Muscle Cells | T0557

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 whole gene

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Coronary Arteries

Organ Type:

Blood Vessel

Growth Properties:

Adherent

Morphology:

Multipolar

Passage Number:

N/A

Population Doubling:

35-40 hours

Seeding Density:

10,000 - 20,000 cells/cm10²; recommended split ratio of 1:2 to 1:4

Markers:

Smooth Muscle Actin

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm. to a final concentration of 5% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify the transgene expression.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C