abm | Immortalized Human Epidermal Keratinocytes (SIK) | T0770

abm | Immortalized Human Epidermal Keratinocytes (SIK) | T0770

Immortalization Method:

Spontaneous immortalization

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Foreskin

Organ Type:

Skin

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 5% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Chromosomal analysis; 2) Immunoblotting of cell cycle control proteins; 3) Telomerase activity using telomeric repeat amplification protocol

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C