abm | Immortalized Human Glomerular Epithelial Cells (HGEC) - SV40 | T0694
- SKU:
- T0694
- Availability:
- 5 to 7 Days Shipment
- Size:
- 1x10⁶ cells / 1.0 ml
Description
abm | Immortalized Human Glomerular Epithelial Cells (HGEC) - SV40 | T0694
Immortalization Method:
Immortalized by transfection with SV40-T and H-ras
BioSafety Level:
II
Organism:
Human
Species:
Human
Source Organ:
Kidney
Organ Type:
Kidney
Growth Properties:
Adherent
Morphology:
Cobblestone
Passage Number:
N/A
Population Doubling:
30 hours
Seeding Density:
N/A
Markers:
PHM5, CALLA and cytokeratin
Donor Age:
1 month old
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow II medium, available at abm. to a final concentration of 10%, HEPES to a final concentration of 10 mM, L-glutamine to a final concentration of 2 mM, and Penicillin/Streptomycin solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
1) Immunofluorescence
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C