abm | Immortalized Human Glomerular Mesangial Cells (T-HMC) | T0695

abm | Immortalized Human Glomerular Mesangial Cells (T-HMC) | T0695

Immortalization Method:

Immortalized by transfection with SV40-T and H-ras

BioSafety Level:

II

Organism:

Human

Species:

Human

Source Organ:

Kidney

Organ Type:

Kidney

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

t-PA, PAI1, u-PA, IL-6, and Prorenin

Donor Age:

1 month old

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium, available at abm. to a final concentration of 10%, HEPES to a final concentration of 10 mM, L-glutamine to a final concentration of 2 mM, and Penicillin/Streptomycin solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Immunofluorescence

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C