abm | Immortalized Human Lens Epithelial Cells - SV40 | T0573

abm | Immortalized Human Lens Epithelial Cells - SV40 | T0573

Immortalization Method:

Serial passaging and transduction with recombinant adenovirus carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Infant lens

Organ Type:

Eye

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

76 hours

Seeding Density:

5,000 - 10,000 cells/cm10²; Recommended split ratio is 1:2

Markers:

None

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

The Immortalized Human Lens Epithelial Cells react positively with the monoclonal antibody to SV40 large T antigen. Western blot and precipitation are used to confirm the presence of βH- and γ-crystallin, the proteins that are markers for cell differentiation in the lens epithelium. SV40 was observed through IHC (Figure 6).

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C