abm | Immortalized Human Mammary Fibroblasts (HMF3A) | T0153

abm | Immortalized Human Mammary Fibroblasts (HMF3A) | T0153

abm. These conditionally immortalized cells are suitable for cell engineering and are theoretically capable of generating immortal diploid human lines.

Immortalization Method:

Combined transduction with retrovirus carrying a temperature sensitive mutant (U19tsA58) of the SV40 Large T Antigen and a catalytic subunit of human telomerase (hTERT)

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Mammary Tissue

Organ Type:

Breast

Growth Properties:

Adherent

Morphology:

Spindle shaped

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, 1% L-glutamine , and Penicillin/Streptomycin Solution to a final concentration of 1%
Carbon dioxide (CO₂): 5%, Temperature: 33.5°C.

Quality Control:

1.) Telomerase-detection assay was used to detect telomerase activity. 2.) Flow cytometry confirmed expression of T antigen.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C