abm | Immortalized Human Oral Mucosal Epithelial Cells (hTERT-OME) | T0043

abm | Immortalized Human Oral Mucosal Epithelial Cells (hTERT-OME) | T0043

Immortalization Method:

Transfection with the retroviral vector (pLXSN–hTERT)

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Gingival mucosa

Organ Type:

Oral

Growth Properties:

Adherent

Morphology:

Spindle shaped

Passage Number:

N/A

Population Doubling:

25 - 35 hours

Seeding Density:

10,000 - 20,000 cells/cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available from abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, 200 µg/ml G418 (G271), and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Immunocytochemistry showed the cells expressed cytokeratin and vimentin; 2) Western blot and RT-PCR detected the presence of hTERT

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C