abm | Immortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5) | T0752
- SKU:
- T0752
- Availability:
- 5 to 7 Days Shipment
- Size:
- 1x10⁶ cells / 1.0 ml
Description
abm | Immortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5) | T0752
Immortalization Method:
Immortalized with the vector pRetro-E2 containing SV40 large T antigen
BioSafety Level:
II
Organism:
Homo sapiens
Species:
Human
Source Organ:
Cartilage
Organ Type:
Skeletal
Growth Properties:
Adherent
Morphology:
Fibroblast-like
Passage Number:
N/A
Population Doubling:
N/A
Seeding Density:
N/A
Markers:
ALCAM (CD166)
Donor Age:
Adult
Donor Gender:
Pooled from male and female OA patients
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, HEPES to a final concentration of 100 mM, L-glutamine to a final concentration of 2 mM, ascorbic acid to a final concentration of 0.1 mM, Sodium Pyruvate Solution to a final concentration of 0.1 mM, and L-glucose to a final concentration of 2.7 μM.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
1) qPCR; 2) Flow cytometry; 3) Differentiation assays; 4) Immunocytochemistry
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C