abm | Immortalized Human Prostate Epithelial Cells (EP156T) - hTERT | T0728

abm | Immortalized Human Prostate Epithelial Cells (EP156T) - hTERT | T0728

in vitro model for the prostate, offering a tool for the study of not only normal development and physiology, but also the steps leading to malignancy.

Immortalization Method:

Immortalized with retrovirus containing hTERT.

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Prostate

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Epithelial

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

2500 cells/cm10²

Markers:

Puromycin resistance, cytokeratin 5/6/7/8/14/18, p63, estrogen receptor-β, androgen receptor

Donor Age:

66 years old

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is MCDB 153 medium (Sigma). To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 1%, Penicillin/Streptomycin Solution to a final concentration of 1%, MEM Non-essential Amino Acid Solution to a final concentration of 1X, bovine pituitary extract (Gibco) to a final concentration of 50 µg/mL, insulin (Sigma) to a final concentration of 5 ug/mL, transferrin (Sigma) to a final concentration of 5 ug/mL, EGF (Sigma) to a final concentration of 5 ng/mL, hydrocortisone to a final concentration of 200 nM, triiodothyronine (Sigma) to a final concentration of 10 nM, testosterone (Sigma) to a final concentration of 10 nM and sodium selenite (Sigma) to a final concentration of 5 ng/mL.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Western blot; 2) qRT-PCR; 3) Three-dimensional cell culture

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C