abm | Immortalized Human Prostate Stromal Cells (PM151T) - hTERT | T0727

abm | Immortalized Human Prostate Stromal Cells (PM151T) - hTERT | T0727

in vitro model for the prostate, offering a tool for the study of not only normal development and physiology, but also the steps leading to malignancy.

Immortalization Method:

Immortalized with retrovirus containing hTERT.

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Prostate

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Fibroblast

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

2500 cells/cm10²

Markers:

Puromycin resistance, progesterone receptor, estrogen receptor-β, androgen receptor, smooth muscle-myosin heavy chain, calponin, SM22, smooth muscle α-actin, myocardin

Donor Age:

62 years old

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is MCDB 131 medium (Sigma). To make the complete growth medium, add the following components to the base medium: decalcified horse serum (Sigma) to a final concentration of 15%, pH 7.2 HEPES to a final concentration of 10mM, MEM Non-essential Amino Acid Solution to a final concentration of 1X, insulin (Sigma) to a final concentration of 5 µg/mL, transferrin (Sigma) to a final concentration of 10 µg/mL, estradiol to a final concentration of 10 nM, dexamethasone (Sigma) to a final concentration of 100 nM, sodium selenite (Sigma) to a final concentration of 5 ng/mL, basic FGF (Sigma) to a final concentration of 1 ng/mL and EGF (Sigma) to a final concentration of 0.1 ng/mL.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated serum for cell culture unless specified otherwise.

Quality Control:

1) Western blot; 2) qRT-PCR; 3) Transforming growth factor-β/endothelin A treatment

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C