abm | Immortalized Human Proximal Tubule Cells (HPCT-03ts) | T0748

abm | Immortalized Human Proximal Tubule Cells (HPCT-03ts) | T0748

Immortalization Method:

Immortalized with retrovirus containing temperature-sensitive SV40 large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Kidney

Organ Type:

Renal

Growth Properties:

Adherent

Morphology:

Epithelial-cobblestone

Passage Number:

N/A

Population Doubling:

24 - 48 hours

Seeding Density:

150,000 cells/cm10²

Markers:

NBCe1-A, NHE3, G418 resistance

Donor Age:

75 year old

Donor Gender:

Female

Donor Ethnicity:

N/A

Propagation:

Cells are grown on Millicell Cell Culture Insert, 12 mm, hydrophilic PTFE, 0.4 µm (Millipore) that are lightly coated with a 20% dispersion of rat-tail collagen (in 60% ethanol) plus 1 µg/mL laminin.
The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 5%, HEPES (Sigma) to a final concentration of 15 mM, sodium selenite (Sigma) to a final concentration of 20 nM, ascorbic acid-2-phosphate (Sigma) to a final concentration of 50 mM, transferrin (Sigma) to a final concentration of 5 µg/mL, Insulin Solution to a final concentration of 5 µg/mL, mouse EGF (Sigma) to a final concentration of 10 ng/mL, dexamethasone to a final concentration of 4 µg/mL, T3 (Sigma) to a final concentration of 1 nM, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days by first removing it from the bottom of the filter and then from the top. Add fresh medium first to the top and then to the bottom.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C. To differentiate cells, increase temperature to 38.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Western blot

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C