abm | Immortalized Human Skin Fibroblast - SV40 | T0302

abm | Immortalized Human Skin Fibroblast - SV40 | T0302

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Skin

Organ Type:

Skin

Growth Properties:

Adherent

Morphology:

Bipolar

Passage Number:

N/A

Population Doubling:

15-28 hrs

Seeding Density:

15,000-25,000 cells/cm10²; Recommended split ratio is 1:2 to 1:3

Markers:

N/A

Donor Age:

43 years old

Donor Gender:

Female

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C